Fig. 9. (A) Exogenous FGFs and BMPs modulate SMG morphology. A range of concentrations of exogenous growth factors were added to glands and cultured for 20 hours. An example of each is shown, FGF1 (100 ng/ml), FGF2 (100 ng/ml), FGF7 (200 ng/ml), FGF10 (500 ng/ml), BMP7 (100 ng/ml), and BMP4 (100 ng/ml), were added to glands and cultured for 20 hours. The gland with BMP4 is also shown after 42 hours in culture, and resembles a SU5402-treated gland. (B) FGF7, FGF10 and BMP7 rescue SMGs treated with SU5402 (1.5 µM IC50). The addition of FGF7 (500 ng/ml), FGF10 (500 ng/ml), or BMP7 (100 ng/ml) rescues SMGs treated with SU5402 (1.5 µM=IC 50). FGF1 (100 ng/ml) is not able to rescue the glands. The number of buds=(T42/T2), the number of buds at 42 hours/the number of buds at 2 hours. (C) An image of a gland from each group is shown after 44 hours of culture.

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