Fig. 5. Rescue of the so¹ mutant by the different mutated so10 enhancers. (A) Schematic drawing of the Gal4 system used for the rescue experiment. Flies expressing the Gal4 gene under the control of the different so10 enhancers were crossed to flies carrying the UAS promoter controlling the so cDNA. The crosses were all carried out in a so¹ mutant background. (B,C) Rescue of the so¹ mutant eye phenotype with so10 and so10^TOYmt, respectively. (D) Rescue obtained with the so10^EY/TOYmt enhancer. The eye size shown is the largest obtained. The variations in size were reminiscent of those observed in an ey² mutant with one eye missing. (E) No rescue was observed with the so10^TOY+EYmt. (F) Schematic drawing of the Gal4 system used to rescue the ey null mutant. (G,H) Rescue of the ey null mutant ey^J5.71 with the ey-enhancer Gal4. Both UAS-ey (G) and UAS-toy (H) were able to rescue the ey^J5.71 mutant when driven with ey-Gal4. In both cases (G,H), the eyes were almost normal in size for all of the flies analysed. The ey^J5.71 null mutant showed an eye reduction of between 50-75% for 20% of the flies (I), and 75%-100% for 80% of the flies (J) (Callaerts et al., 2001).

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