Fig. 4. Galectin 1 expression in the developing testis. In situ hybridisation of testis sections with 35S-labelled antisense probes and Toluidine Blue counterstaining (A,C-N), or anti-galectin 1 alkaline phosphatase immunohistochemistry (B). (A,B) Adjacent serial sections of P7 wild-type testis showing expression of galectin 1 mRNA (A) and protein (B) in testis cords (c), tunica albuginea (t), interstitial cells (arrows), peritubular cells (arrowheads) and blood vessel (v). Note the variable intensity of mRNA and protein staining in the individual testis cord cross sections, and the localisation of signal in the Sertoli cell cytoplasm at the centre of the cords. (C,D) Brightfield and darkground images of P4 testis section probed for Wilm’s tumour (Wt1) mRNA. Signal intensity appears uniform over all testis cords. (E,F) Newborn (P0) testis showing localised differential expression of galectin 1 in the cords. (G,H) E18 testis showing galectin 1 expression in a discrete region of testis cord (arrows). (I,J) E16 testis, showing uniform signal intensity in all testis cords. (K,L) Consecutive serial sections of P8 XXSxra testis, probed for galectin 1 and Amh, respectively. (M,N) Longitudinal stretch of P8 XXSxra testis cord showing junction between regions of low and high galectin 1 mRNA expression. Autoradiographic exposures (in days) were 4 (A,E,F), 9 (C,D), 18 (G-J) and 3 (K-N).
