Fig. 4. Pdgfra+ OLPs are generated from the Olig2+ pMN domain in mouse. (A,B) Sections from E10.5 mouse spinal cord were subjected to in situ hybridization with Nkx2.2/Olig2 (B) or Olig2/Irx3 (A) probes. Nkx2.2 expression is flanked by Nkx2.2 ventrally and Irx3 dorsally. (C) Double labeling of E12.5 spinal cord with anti-Nkx2.2 (green) and anti-Olig2 (red) antibodies by immunofluorescence. (D-F) E12.5 spinal cord were double-labeled by in situ hybridization with Olig2/Pdgfra (D,E) or Nkx2.2/Pdgfra (F). D,E are from two separate embryos. Pdgfra expression is indicated by arrows.
