Fig. 6. TGFß2 treatment enhances outflow tract apoptosis in whole mouse embryo culture. Embryonic day 11.5 wild-type embryos were treated with 0.1-30 ng/ml TGFß2 protein and placed in whole embryo culture for 24 hours, then processed as described in Materials and Methods. Wild-type (Control) or mutant (Rxra–/–) E11.5 embryos were also cultured for 24 hours as comparative controls. TGFß2 treatment enhanced outflow tract apoptosis in a concentration-dependent manner. Apoptosis was quantitated using the TUNEL assay as described in Fig. 5. Dextrodorsalconal cushion (white bars). Sinistroventralconal cushion (black bars). *P<0.05, compared with control DDCC. **P<0.05, compared with the respective DDCC and SVCC Control and 0.1 ng/ml TGFß2. Error bars indicate s.e.m.
