Fig. 2. Tkv^Q253D-expressing cells proliferate and grow faster than do wild-type cells. (A) Area definition for doubling time and clone area measurement experiments. dpp expression is indicated by the black line down the center of the disc. For wing definition, the longest fold along the AP axis was used as a border. For mediolateral definition, the most lateral folds were used. Clones exterior to them were considered lateral clones, and clones interior to them were considered medial clones. (B) Cell doubling times of Tkv^QD-expressing cells and control cells expressing GFP alone. The caspase inhibitor p35 was co-expressed in both experiments. Clones were scored in the entire wing (total), and in medial and lateral areas. Numbers of clones counted in each area are noted inside the bars. Black bars represent the control experiment and gray bars the Tkv^QD samples. Numbers on top of each bar correspond to the cell doubling time. P values are noted below each category. Tkv^QD cell doubling time is significantly reduced and lateral cells are dividing with a 20% shorter cell cycle (2.7 hours shorter, from 13.5 to 10.8 hours doubling time). (C) Clone size of Tkv^QD and wild-type Flip-out/Gal4 clones shown as pixels per clone. Nomenclature and color code as in B. Numbers on top of the gray bars correspond to the increase in Tkv^QD clone size compared with control clones. Tkv^QD clones are significantly larger than control clones and lateral clones are more than 3.5 times larger. (D) S-phase cells visualized by BrdU incorporation in Tkv^QD Flip-out/Gal4 clones. Left panel shows clone position by GFP signal and right panel shows BrdU incorporation. Lateral clones show a strong BrdU incorporation.

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