Fig. 4. tkv mutant cells are proliferation and growth impaired. (Left panels) Confocal images of mitotic recombination clones of the indicated genotype visualized by GFP. A Minute background was used. GFP– cells correspond to the experimental populations, wild-type or tkv7 cells. Cells in the same disc expressing GFP and therefore M–/+ (wild-type experiment) or M–/+ tkv7/+ (tkv7 experiment) were used as an internal controls. tkv7 cells colonize much smaller an area than do wild-type cells and they are found mainly in the lateral areas (arrows, compare GFP– population in wt and tkv7 experiments). Some tkv7 discs show an abnormal morphology (lower panel in tkv7 experiment). Scale bar: 100µm. (Right panels) FACS analysis of the same experiment as in left panels. Gray filled traces correspond to the internal GFP+ control cells, and black traces correspond to the GFP– experimental populations. Numbers in the top right-hand corner in the size histograms show the ratio of the mean size of GFP– cells (experimental population) versus the mean size of GFP+ cells (control population) from FSC data. tkv7 cells show a severe reduction in the S phase and G2 populations, and an increase in G1 population (DNA content histogram). Cell size is not affected. tkv7 has the same effects in notum and wing regions (data not shown).
