Fig. 1. All neurons of the NB7-3 lineage can be identified individually. (A) The NB7-3 lineage as revealed by DiI labeling (Bossing et al., 1996): three interneurons (arrows) with contralateral projections across the posterior commissure (thick arrow) and one motorneuron (arrowheads). Anterior is upwards; broken line indicates the midline. (B) Schematic representation of the NB7-3-derived neurons with their designations. Anterior is upwards; P, posterior commissure; A, anterior commissure. (C,D) Dorsal view of the VNC of an egGal4::GFP (C) and a wild-type (D) L1 larva. Anterior is upwards. Crz is stained in red, while GFP (C) and Serotonin (D) are stained in green. One Eg-positive NB7-3 derived neuron co-expresses Crz in the segments T2 to A6 (yellow in C; arrows). This neuron lies in most cases lateral to the serotonergic neurons (D, arrows) in the EW3 position. (E) NB7-3 lineage development showing markers and genealogy of the lineage. The markers listed are expressed in the postmitotic neurons as indicated by each bracket. 5-HT, Serotonin; Crz, Corazonin; DDC, Dopa-decarboxylase; dSERT, Drosophila Serotonin transporter; Eg, Eagle; En, Engrailed; Hb, Hunchback; Hkb, Huckebein; Isl, Islet; Pdm-1, POU-domain protein 1; TrpH, Tryptophan hydroxylase; Zfh-1 and Zfh-2: Zinc-finger homeodomain 1 and zinc-finger homeodomain 2; PCD, programmed cell death; NB, neuroblast; GMC, ganglion mother cell.
