Fig. 3. hb and pdm1, but not cas, are expressed in the NB7-3 lineage. Flat preparations of wild-type embryos. Dorsal views, anterior is leftwards; broken line represents the midline; arrowheads indicate GW. (A) Three hemineuromeres of a stage 12 embryo stained for Hb (green) and Eg (red) showing different phases of NB7-3 lineage development in each hemisegment. Left position: Hb is expressed in NB7-3 (curved arrow) before the generation of progeny. Right position: two Hb-positive cells are detected, i.e. NB7-3 and GMC7-3a (long arrows). Medial position: three Hb-positive cells are seen corresponding to NB7-3 plus the early progeny (short arrows). At stage 15 (B-D), two of the four Eg positive neurons (green) in the NB7-3 lineage are Hb positive (yellow, B). These are always GW (arrowhead) and EW1 (arrow). Hb is shown in red. (C) Pdm1 (green) in the NB7-3 lineage is very variable in stage 15 embryos and is detectable in up to all four Eg-positive neurons (yellow) of the lineage. (D) Cas (green) is not detected in the NB7-3 progeny neurons (red).
