Fig. 5. hb affects Serotonin and Corazonin synthesis in the NB7-3 lineage. Ventral nerve cords of L1 larval stage; dorsal view; anterior is upwards. (A-C) anti-Crz; (D-F) anti-Serotonin. (A) In wild-type hemisegments, T2 to A6 show one corazonergic EW3 neuron each (arrows). (B) In the hb9 allele, Crz (arrows) is missing in a significant number of hemisegments (asterisks). (C) Overexpression of hb leads to a complete loss of Crz. (D) In wild-type segments, T2 to A7 show two serotonergic neurons in each hemisegment (arrows), whereas the last segment shows only one serotonergic neuron. (E) In the hb9 mutant a significant number of hemisegments shows only one serotonergic neuron (arrows). (F) Overexpression of hb yields often three to four serotonergic neurons per hemisegment (arrows).
