Fig. 4. Dominant-negative behavior of Dx^PRM. (A-D) Adult wings. (E,F) Wing discs of third-instar larvae. SOPs are shown in green, and Deltex derivatives are shown in red. (G-I) Wing discs of third-instar larvae. Wg and Deltex proteins are shown in green and red, respectively. (A,C,E) Co-expression of Dx^full and Dx^PRM. Note that phenotypes induced by either Dx^full or Dx^PRM were suppressed by co-expression of both proteins (see Fig. 3B,D,H,J,N,P). (B,D,F) Overexpression of Dx^NBS-PRM with the ptc-Gal4 driver. Note that overexpression of Dx^NBS-PRM did not result in the wing-notch phenotype. (G) Endogenous expression of Wg (green) was detected along the boundary of the dorsal/ventral compartments in the wild-type wing discs of third-instar larvae. (H) Overexpression of Dx^PRM. Endogenous expression of Wg (green) was suppressed in the cells expressing Dx^PRM (red). A high-magnification photograph is shown at the top right. (I) Overexpression of Dx^NBS-PRM. Note that Dx^NBS-PRM (red) did not suppress the Wg (green) expression. A high-magnification photograph is shown at the top right.

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