Fig. 2. Loss of rasp activity does not affect Wg signaling. (A-F) Confocal micrographs of late third instar wing imaginal discs containing rasp7F21 (rasp–) clones. In this and all subsequent micrographs, anterior is upwards and dorsal is on the left. Large rasp– clones that cross the DV boundary were generated in a Minute heterozygous (M–/+) background and are marked by the absence of green fluorescent protein (GFP). (C,F) Broken line marks the approximate position of the DV boundary. (A) The absence of GFP in green marks the tissue homozygous for rasp. (B) The double row (DR) of anti-Sc staining shown in red marks the anterior proneural region along the presumptive wing margin. Activation of proneural gene expression along the anterior margin depends on Wg signaling. (C) Overlay of A and B. Note that anti-Sc levels and pattern within the clone are not affected by loss of rasp. (D) Absence of GFP in green marks tissue homozygous for rasp. (E) Dll is normally expressed throughout the majority of the presumptive wing pouch and is delimited by a broken line. Activation of Dll expression in the wing pouch depends on Wg signaling. (F) Overlay of D,E. Note that anti-Dll levels within the clone are not affected by loss of rasp.
