Fig. 6. Hh transcription and protein levels do not require rasp. (A-F) rasp^7F21 (rasp^–) clones in wing imaginal discs. (A-C) Analysis of hh transcription in rasp^– clones. (A) Clone is marked by the absence of GFP. (B) Anti-ß-gal staining in red. The hh^P30 enhancer trap is a reporter of hh transcription. The level of hh transcription in posterior cells is not altered by the absence of rasp. (C) Overlay of A and B. (D-F) Analysis of Hh protein levels in rasp^– clones. (D) Clones are marked by the absence of GFP. (E) Anti-Hh protein shown in red. There is no detectable increase or decrease in Hh protein levels in the absence of rasp. (F) Overlay of D and F.

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