Fig. 4. acr-5 expression in DB neurones is induced by VAB-7 repression of unc-4. (A-D) acr-5::gfp in lateral views of L1 larvae. (A) acr-5::gfp is expressed in wild-type DB motoneurones but is absent (B) from DBs in a vab-7(e1562) mutant. (C) acr-5::gfp is ectopically expressed in DA neurones of an unc-4(e120) mutant. (D) acr-5::gfp expression is restored to the DBs by genetic removal of unc-4 in the unc-4(e120);vab-7(e1562) double mutant. (E) acr-5::gfplacZ detected by anti-ß-galactosidase antibody in an adult hermaphrodite expressing ectopic VAB-7 from weIs10. acr-5::gfplacZ, which is normally only in DBs and VBs, is induced in DAs, VAs and ASs. (F) unc-17::gfp expression of animal in E used to identify the neurones. Anterior is towards the left. Scale bar: 10 µm.
