Fig. 10. Notch activation is necessary and sufficient for Hairy2 expression in the ectoderm, but Su(H)/RBP-J activity is not required. Electroporation of GFP + Su(H)^DBM was performed on stage 5 embryos, and the embryos were cultured for 9 hours until stage 9 (A,B). Slug expression is not affected (B). Electroporation of GFP+CNIC^C89 (C,D) or GFP+Delta1^stu (E,F) was performed on stage 5 embryo, and the embryos were cultured for 4 hours until stage 7. Transfection of CNIC^C89 has no effect on Hairy2 expression in the epidermis (D), while ectopic Hairy2 expression in the neural plate is observed (D, arrows in inset). Transfection of Delta1^stu reduced the level of Hairy2 expression in the epidermis (F, arrowheads)

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