Fig. 4. Forced activation of Notch signaling in the ectoderm represses expression of head-fold markers. Electroporation of GFP (A,B,G,H), GFP+CNIC (C,D) and GFP+CNIC
C89 (E,F,I,J) was performed on stage 6 embryos, and the embryos were cultured for 7 hours until stage 9. Transfected areas are visualized by the fluorescence of GFP overlaid on bright field image of electroporated live embryos (A,C,E,G,I). Slug and Pdgfr
expression in the head fold (B,D,F,H,J) are shown at higher magnification, corresponding to the boxed areas of A,C,E,G,I, respectively. Forced activation of Notch signaling by CNIC and CNICC89 (cytoplasmic domain of chicken Notch1) reduced the expression of Slug and Pdgfr in the head fold (arrowheads in D,F,H).
