Fig. 5. pou2 mRNA injection rescues spg mutants. (A-H) Injections of pou2 mRNA and lacZ mRNA. The dorsal midline of the injected embryos is indicated by a white arrowhead, to show the unilateral injection of lacZ/pou2. (A-D) pax2.1 staining. Wild-type embryo showing laterally expanded pax2.1 expression on the injected side. (B-D) spghi349/spghi349 mutant embryos injected into one cell of a two-cell stage embryo with pou2 and lacZ mRNA and fixed at the three-somite stage. pax2.1 staining is purple, lacZ staining in brown. (B,C) pax2.1 expression at the MHB is clearly rescued on the injected side of the embryo. (D) Dorsoposterior view of a mutant embryo, showing pax2.1 staining in the intermediate mesoderm. pax2.1 expression is not affected in this tissue. (E,F) Lateral expansion of wnt1 after pou2 mRNA injection in the wild-type embryo (E) and rescue of expression in the mutant embryo (F). (G,H) Rescue of krox20 expression after pou2 mRNA injection.
