Fig. 1. Developmental regulation of TBP and TLF. (A) RNase protection experiments. The RNA used is indicated above each lane and the detected transcripts identified are listed to the right of each panel. Lane 1 shows a control with only tRNA. (B) The origin of the RNA, pachytene spermatocytes (spermatocytes), and haploid spermatids (spermatids) is shown above each lane and the transcripts are listed to the right of each panel. (C) Immunodetection of TBP, TLF and CREM. The stage-specific protein extracts used are indicated above each lane and the locations of TBP, TLF and the CREM isoforms are indicated to the right of each panel.
