Fig. 5. Gug– tissues affect the protein distribution detected by antisera raised against the Gug protein. Anti-Gug antibody stains to embryos at blastoderm (A,C) and early gastrula stages (B,D). Wild-type (A,B) and embryos derived from Gug35 germline clones (C,D). Note that for the lower panels, staining is absent from the nuclei unlike in wild type (arrow, A). The asterisk in B indicates one of several mitotic domains where Gug is lost. Note that staining of embryos with the relevant pre-immune serum when overstained gives similar results to that shown in D. Clones of cells homozygous for GugS2 in imaginal leg discs, detected by the absence of GFP (E), show reduced levels of staining with anti-Gug antibody (F). Clones of cells that lack Gug+ products were induced at 48-72 or 96-120 hours after egg laying. Ventral is towards the bottom.
