Fig. 6. Gug– clones affect ventral-specific patterning and morphogenetic events in legs. Clones were induced at 48-72 or 72-96 hours after egg laying and are marked by the yellow bristle marker (highlighted by the broken blue lines). (A,B) Anterodorsal clones in the femur (fe) showing normal morphology (A) and higher density of bristles (B) compared with a wild-type leg. (C) A first leg with a small posterolateral Gug– clone with little effect on leg patterning, although showing a higher density of bristles than normal. Arrowheads indicate the large bristles formed in a wild-type first leg femur, in a ventral position in the posterior compartment. (D) A posterior Gug clone in the ventral region of the femur. The large bristles do not form (arrow; compare with C). (E) Ventral clone in the second tarsal segment of a second leg shows the deletion of a spur bristle (arrow). In wild type, each tarsal segment has two spur bristles at the distal end (arrowheads). (F) First leg carrying a Gug– clone. The transverse rows (arrowhead; compare with the wild type in G) and the sex comb (arrow) in the basitarsus are not formed in the clone. In wild type (G), the sex comb carries 10-12 specific bristles. Here, only four bristles are made, deriving from wild-type tissue. (G) Wild-type basitarsus of a first leg, showing the ventral transverse rows. (H) Antero- and posteroventral Gug– clones in the femur-tibia region lead to fusion of these leg segments.
