Fig. 4. Expression patterns of dpy-11 and subcellular localization of its product. (C,E,G) Nomarski images of D,F,H, respectively. (A) dpy-11 promoter-driven gfp reporter with a nuclear localization signal in L3 larva. (B) Cytosolic gfp reporter in L4 larva showed no seam cell expression (arrowhead). (C,D) The DPY-11 tagged with GFP at the C-terminus (pd11CTGFP) presented a heterogeneous cytosolic expression pattern in the hypodermis around the vulva. (E,F) The fusion transgene encoding full-length DPY-11 with a GFP tagged between the transmembrane domain and the C-terminus (pd11AGFPC) presented a heterogeneous cytosolic expression pattern in the body hypodermis. (G,H) In gastrulating embryos, cytosolic expression (pd11CTGFP) was detected in unfused hypodermal cells (arrows) with membrane association detected in some dying cells (arrowheads). Scale bars: 100 µm (A,B); 10 µm (C-H).

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