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Fig. 6. Expression and purification of the GST fusion construct. WT represents the GST fusion product of the TRX-domain from amino acids 19-133 of DPY-11. G76E is a similar fusion protein with the glycine at position 76 changed to glutamic acid. GST is the expression of an empty pGEX-2T GST vector, which produced only the GST tag.





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