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Fig. 6. Cellular aberrations of dorsal closure in DMBS mutant embryos and in embryos overexpressing DRhk+. The wild-type embryos (A,D,G,I), DMBS mutant embryos (B,E,H,J) and embryos overexpressing DRhk+ (C,F) were stained with an anti-phospho-tyrosine antibody (A-C), phalloidin (D-F), an anti-myosin heavy-chain antibody (G,H), and an anti-phospho-MRLC antibody (I,J). The DMBS mutant embryos were obtained from a mating between DMBSP2/Df(3L)th117 females and DMBSE1/TM3, P[ry+t7.2=HZ2.7]DB2 males, and the mutant embryos were identified by use of the blue balancer. DRhk+ was driven by 69B-GAL4. The aberrantly distributed phospho-MRLC is indicated with arrowheads in J. Polygonally shaped leading edge cells (B,C) and the leading edge cells with aberrant accumulation of F-actin (E,F) are boxed.





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