Fig. 1. (A) Genomic organization of the rn region. Insertion site of the three P elements is denoted by open triangles. The deletion in rn^2–2 is denoted by the extended line. Fragment D was isolated in the previous study (Agnel et al., 1989) and used to initiate the screen for rn. Putative promoters are depicted as angled arrows. The rn and roe transcripts are outlined and the ORFs designated by black boxes for both genes. The ZF domain is represented by gray shading. Deletions used in this study are indicated at the bottom and breakpoints, where known, are shown. Data for rn¹⁹ and rn²⁰ are based on previous studies (Agnel et al., 1989). rn¹⁶ was described as a smaller deletion mapping to the 3' area (Agnel et al., 1989) but our work shows that it extends further, deleting both the common ZF coding exons and the roe-specific exons (not shown). The roe³ mutation (asterisk), is a glutamine to an amber stop codon. (B) Predicted protein structure of Rn and Roe. The N-termini are unique but the C-termini, containing most of the ZF domain, are identical. The glutamine, serine and alanine stretches are designated Q, S and A, respectively. (C) Comparison of Rn with other ZF proteins. Rn has a few close homologs in Drosophila (D.m.), C. elegans (C.e.) and rat. Numbers in circles are the percentage of identical amino acids between Rn and the other proteins in the ZF domain. Rn, Roe and Drosophila CG5557 further share a C-terminal region of homology not present in the other proteins (gray).

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