Fig. 2. Cellular analysis of lola midline phenotype. Wild-type and mutant embryos were fixed and stained with the indicated antibodies. (A,B) Ventral views of the axonal projections of Apterous-positive interneurons, visualized by anti-ß-galactosidase staining of wild-type (A) or lolaORC46/ORE76 (B) embryos bearing a tau-lacZ fusion gene under control of a fragment of the apterous promoter. Note that ß-gal positive axons do not cross the midline (triangle) in wild type, but do so in the mutant (arrows). (C) Ventral view of an anti-Wrapper staining of a lolaORC46 mutant embryo. Note presence of immunopositive midline glial cells (arrow) in every segment. Anterior is towards the top in all three panels.
