Fig. 5. lola interacts genetically with slit and robo. Embryos that were either (A) lola^ORE120/lola^ORE120, (B) slit^IG107/+, (C) lola^ORE120slit^IG107/lola^ORE120 or (D) lola^ORE120robo^z14/lola^ORE120 were fixed, stained with anti-Fasciclin II and visualized by peroxidase histochemistry. Midline crossing of immunoreactive nerve bundles (arrows) was extremely rare in lola^ORE120 embryos and in slit heterozygous embryos (or robo heterozygous, not shown), but very common in embryos that were simultaneously slit or robo heterozygous and lola^ORE120 homozygous (see Table 2 for quantitation). All panels show ventral views of the CNS of stage 17 embryos; anterior is towards the top.

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