Fig. 1. Transgenic analysis of Ubx proximal promoter mutations. Cartoons of promoter constructs tested are on the left and indicate the mRNA start site at +1 (arrow), the number of factor binding sites present at the proximal promoter (P) of each construct, and the position of the ß-galactosidase reporter gene (lacZ). Representative pictures of stage 13-14 embryos showing the pattern of ß-galactosidase expression from each transgene are to the right. The normal anterior and posterior boundaries of Ubx expression are marked with arrowheads. (A) 22UZ Native; (B) 22UZ Deletion; (C) 22UZ GAGA; (D) 22UZ ZESTE; (E) 22UZ NTF-1; (F) 22UZ GAGA/NTF-1; (G) 22UZ ZESTE/NTF-1.
