Fig. 3. The effect of RBF-280 expression on E2F target gene expression and on mitosis. (A-D) In situ hybridization of wild-type and GMRRBF-2804 eye discs with antisense probes to PCNA allows for visualization of E2F target gene expression. (A,C) Low magnification showing whole eye discs. (B,D) High magnification view showing the details of the PCNA expression. (E-H) Anti-phospho-Histone H3 antibody staining of eye discs from wild-type and GMRRBF-2802 eye discs labels cells in mitosis. (E,G) Low magnification showing whole eye discs. (F,H) High magnification view showing the details of mitosis. In all the discs, posterior is oriented towards the right. Arrowheads indicate the morphogenetic furrow.
