Fig. 6. RBF-280 inhibits CyclinD/Cdk4 induced ectopic S phase but not the large eye phenotypes in the Drosophila eye. BrdU staining (red) of eye discs with Cyclin D/Cdk4 overexpression (A-C), or RBF-280+Cyclin D/Cdk4 overexpression (D-F) showing RBF-280 blocks CyclinD/Cdk4 induced ectopic S phase. Clones of cells with CyclinD/Cdk4 or RBF-280+CyclinD/Cdk4 overexpression are marked by GFP (green). Arrowheads point to the second mitotic wave and arrows point to GFP marked clones. (G-K) Phenotypes of fly eyes with CyclinD/Cdk4 or RBF-280+CyclinD/Cdk4 overexpression as indicated.
