Fig. 7. RBF-280 inhibits CyclinD/Cdk4 induced growth and proliferation but not activated Ras-induced cellular growth in the proliferating wing disc cells. (A) Distribution of clone size and cell number/clone for the genotype given is shown. Cell doubling times (DT) are shown. Clones were analyzed 43 hours post-induction. Total number of clones analyzed (n): control, 48; Cyclin D/Cdk4, 51; RBF-280, 60; RBF-280+Cyclin D/Cdk4, 47. P*, comparison of clone area or cell number/clone between RBF-280+Cyclin D/Cdk4 and RBF-280. Clones with RBF-280+Cyclin D/Cdk4 expression occupy similar areas (P*=0.68) and have similar number of cells (P*=0.32) as the RBF-280 clones. Comparison of the clone area or cell number/clone between other genotypes, P<0.0001. (B) RBF-280 does not block Ras^V12-induced cellular growth. Clones were analyzed 43 hours post induction. Total number of clones analyzed (n): control, 52; Ras^V12, 47; RBF-280, 46; RBF-280+Ras^V12, 88. P^#, comparison of clone area or cell number/clone between RBF-280+Ras^V12 and RBF-280; P^##, comparison of cell number/clone between Ras^V12 and wild type. Comparison of the clone area or cell number/clone between other genotypes, P0.0001. (C) Examples of cell clones of each genotype are shown as indicated. Clones are marked by GFP (green). DAPI staining that labels the nuclei is shown in blue. Cell sizes were estimated by the average of clone area/cell number per clone for all genotypes. The ratio between cell sizes of indicated genotype over wild-type cell size is shown.

Return to article