Fig. 2. Survival of ras– clones in the prospective wing blade, wing hinge, lateral notum and medial notum. (A) Subdomains of the wing imaginal disc: (I) presumptive wing blade, (II) presumptive wing hinge, (III) presumptive lateral notum and (IV) presumptive medial notum (see also Fig. 1B). (B) Histogram showing the survival rates of ras– clones in each of the four territories in the mature third instar wing disc, following clone induction during the first larval instar (see Materials and Methods). ras– clones generally survive less well than their ras+ twin clones. However, survival in the presumptive lateral notum was severely reduced relative to survival in other regions of the wing disc. Bars represent the percentage of ras+ clones associated with a ras– twin clone within a given disc area. n, total number of ras+ clones (with or without a paired ras– clone). Clones were induced at 24-48 hours after egg laying (AEL).
