Fig. 3. EGFR/Ras signaling is required for Iro-C expression. (A-E) ras^– (A-B) and Egfr^ts (C-E) clones, marked by the absence of GFP (green), fail autonomously to express mirr-lacZ (A,C,D, red) and autonomously upregulate expression of tsh-lacZ (B, red) or endogenous Tsh protein (E, red). Wing blade specific vgQ-lacZ expression is also seen (C,D, red). Egfr^ts clones were generated using the Minute technique. Note the round shape of mutant clones (A-C,E) located within the prospective lateral notum when compared with the irregular shape of the clones located elsewhere in the disc. (A,B) ras^– clones generated during first larval instar. (C) Egfr^ts clones generated during second larval instar and raised at the severely restrictive temperature of 31°C. (D-E) Egfr^ts clones generated during first larval instar and raised at intermediate restrictive temperature of 28°C.

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