Fig. 5. Quantification of VIP and ChAT expression in E13/14 sympathetic ganglia. The number of VIP- (A,D) and ChAT- (B,E) positive neurons was determined in cervical and thoracic ganglia infected by LIFRß antisense-RCAS and LIFRß sense-RCAS. The area of neuronal SCG10-positive cells in the sympathetic ganglia section was determined morphometrically, as described in Materials and Methods. The numbers of VIP- (G) and ChAT-positive (H) cells were referred to the SCG10-positive area. Scale bar: 100 µm. Data represent the mean±s.d. of several independent experiments as indicated by the numbers. P<0.0001 (Student’s t-test, pairwise comparison between sense and antisense experiments).
