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Fig. 1. Sequence alignment and phylogenetic tree. (A) The predicted amino acid sequences of the PvuBra fragment has been aligned with a sample of metazoan Brachyury and other T-box genes. Only the T domain is shown. The primers used for PCR amplification are indicated by arrows. (B) Phylogenetic tree (amino acid) of several Brachyury orthologues, reconstructed by quartet puzzling. A sample of other T-box genes has been taken to root the Brachyury subtree (see Materials and Methods).





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