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Fig. 5. TEM in situ hybridization on D. japonica. (A-D) DjPax6A antisense-strand RNA; (F) DjPax6A sense-strand RNA; (E,G) Dj18S antisense-strand RNA; (H) DjSyt antisense-strand RNA. (A) Low magnification of the pigment cup ocellus showing some nuclei of photosensitive cells. The box indicates the figure shown in B. (B) Enlargement of A with clusters of gold particles (arrows) on the cytoplasm. (C) Clusters of gold particles (arrows) on the dendrite and the rhabdomeric region of a photosensitive cell. (D) A cluster of gold particles on the perinuclear cytoplasm of a pigment cell. (E) Clusters of gold particles (arrows) on the nucleolus and the endoplasmic reticulum of a cell. (F) No cluster of gold particles is visible on the cytoplasm of a pigment cell or in the rhabdomeric region of a photoreceptor cell. (G) A large cluster of gold particles (arrows) on the rhabdomeric region of a photoreceptor cell. (H) A cluster of gold particles (arrow) on the perikaryon of a photoreceptor cell. d, dendritic region of the photosensitive cell; n, nucleus; nu, nucleolus; p, pigment cup; pc, pigment cell; r, rhabdomeres. Scale bars: 5 µm in A; 0.5 µm in B-H).





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