Fig. 6. Constitutively active CREB phenocopies hematopoietic defects caused by gain of CaM KIV or loss of BMP function. Embryos were injected with RNAs encoding ß-gal (control), or mutant forms of CREB as indicated. (A) Expression of globin in stage 28 embryos analyzed by whole-mount in situ hybridization. (B) The mean number (± s.e.m.) of WBCs (white bars), RBCs (black bars) and total blood cells (shaded bars) present in three random fields of cytospin blood preparations from at least 30 control or experimental tadpole stage embryos.
