Fig. 4. Central nervous system defects in rpr nulls. (A-D) Anti-CCAP staining reveals persistent neurons in XR38/H99 adults. A and B are ventral views; C and D are dorsal views. (A,C) Ventral ganglia of a 2- to 6-day old XR38/TM6B adult, stained with anti-CCAP. An average of 3 CCAP-positive cells are present in these ganglia. (B,D) Ventral ganglia from 2- to 6-day old rpr null XR38/H99 adult. Black lines delineate the boundaries of the abdominal ganglion that are greatly enlarged in XR38/H99. About 20 CCAP-positive cells are present in these enlarged abdominal ganglia. (E,F) EcR-A-expressing neurons also persist in XR38/H99 adults. Ventral view of the T3 thoracic neuromere and abdominal neuromere of a 1- to 2-day old XR38/TM6B (E) and XR38/H99 adult (F). Black lines delineate the boundaries of the abdominal ganglion. All photos are taken at the same magnification.
