Fig. 7. shot mutants affect E-cadherin (DE-cad) contacts between fusion cells and F-actin associated with these contacts. (A-D) Stage 13, wild type. A E-cadherin (red in D) contact (arrow in A) between two fusion cells (asterisks in B) is associated with an F-actin (blue in D) containing track (arrow in C) spanning the two cells. Membrane (green in D)-associated with the track (arrow in B) bisects the fusion cells. (E-H) Stage 13, shot3. E-cadherin contacts (arrow in E) between fusion cells (asterisks in F) fail to form, and no F-actin rich track is detected (arrow in G). Fusion cells were identified by their position (Figs 2,3). (I-L) Stage 13, shot3. Weak E-cadherin contacts (arrows in I) form between the posterior branch fusion cell (p in J) and a tracheal cell (t) in the anterior branch. No F-actin-rich track is detected (arrow in K). (M-O) Stage 13, shot3. Fusion cells occasionally form aberrantly oriented DE-cadherin contacts (arrow in M). These contacts lack detectable associated F-actin (arrow in O).
