Fig. 9. Interactions between Shot and the cytoskeleton organize the tracheal cell cytoskeleton. (A,B) Stacks of five 1 µm confocal sections through dorsal trunk cells. (A) Stage 15, shot³. Tracheal expression of Shot L(C) restores normal actin-GFP distribution in fusion cells. (B) Stage 16, shot³. Tracheal expression of Shot L(A)-GAS2 restores normal actin-GFP distribution in fusion cells. (C,D) Confocal sections (1 µm) of dorsal trunk cells. (C) Stage 15, shot³. Tracheal expression of Shot L(C) restores apical accumulation of C-Shot L-GFP, a microtubule-binding protein. (D) Stage 15, shot³. Tracheal expression of Shot L(C) restores apical accumulation of C-Shot L-GFP. (E-H) Stage 15, shot³. Dorsal trunk tracheal cells express Shot L(C)-GAS2-GFP, which lacks both cytoskeletal interaction domains. (E) Apical accumulations of F-actin (phalloidin staining) surround blind-ended lumens terminating at an anastomosis site. (F) Microtubules (detected with anti-tubulin) do not accumulate apically in tracheal cells. (G) Shot L(C)-GAS2-GFP distribution. (H) E-G merge. Scale bars: in B, 25 µm for A,B; in D, 10 µm for C,D; in H, 10 µm for E-H.

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