Fig. 1. SOX10+ and NGN2+ cells develop in dNT cultures. (A-E), cultures grown for 48 hours in the indicated concentrations of BMP2 were stained with anti-SOX10 and counter-stained with the nuclear dye Topro-3. (E) The percentage of SOX10+ cells was scored. The data are derived from two independent experiments. Arrows indicate dense reaggregates of SOX10– neural tube cells, arrowheads indicate relatively more dispersed SOX10+ neural crest cells. (F-H) Double-labeling of a section of E10.5 rat spinal cord with anti-SOX10 and anti-NGN2. Arrows indicate NGN2+ cells at dorsal margin of the spinal cord, arrowheads indicate double-positive cells in the neural crest migration pathway. (I-K) dNT culture grown for 48 hours in 10 ng/ml BMP2 double-labeled for SOX10 and NGN2. Arrows indicate double-positive cells.
