Fig. 2. BRN3A+ cells in dNT cultures are peripheral sensory neurons. Cultures grown for 3 days in 10 ng/ml BMP2 were double-labeled for BRN3A (A,D,G) and the indicated markers (B,E,H). NeuN is a pan-neuronal marker; peripherin is a peripheral neuron-enriched marker. Some BRN3A+ cells also co-express RET (not shown). (G-I) The BRN3A+ cells co-expressing NeuN and peripherin were often found in close association with clusters of SOX10+ cells. However the BRN3A+ cells are SOX10– as the latter marker is rapidly downregulated during neuronal differentiation (not shown; L. L., J. Kim and D. J. A., unpublished).
