Fig. 5. NGN1- and MASH1-infected cells that express BRN3A or PHOX2B co-express pan-neuronal and peripheral neuron-specific markers. Cultures infected with the retroviruses indicated above the columns were grown for 3 days in 10 ng/ml BMP2, fixed and triple-labeled with antibodies to BRN3A (A,I) or PHOX2B (B,J); TuJ1 (C,D) or RET (K,L); and GFP (E,F,M,N). Arrows indicate infected cells labeled by all three antibodies. ‘Merge’ indicates superposition of the three overlying panels in the same column. I-P are shown at twice the magnification of A-H.
