Fig. 7. NGN1 induces BRN3A and PHOX2B in non-overlapping cell populations at 25 ng/ml BMP2. NGN1 retrovirus-infected cultures were grown for 3 days in 25 ng/ml BMP2, fixed and triple-labeled with antibodies to BRN3A (A, red), PHOX2B (B, blue) and GFP (C, green). (D,E) The BRN3A⁺, GFP⁺ (arrows) and PHOX2B⁺, GFP⁺ cells (arrowheads) are non-overlapping.

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