Fig. 8. NGN1 and MASH1 both induce autonomic neurogenesis in NCSC clonal cultures. Colonies were grown for 4 days after infection with the retroviruses indicated above the columns, before fixation and labeling. (A-H) Representative colonies from NGN1- and MASH1-infected cultures labeled with antibodies to NF160 (C,D), GFP (A,B) and with DAPI to visualize cell nuclei (E,F); (G,H) Phase-contrast views of the fields shown in A,C,E and B,D,F, respectively. Arrows in B,D,F,H indicate a neuron; arrowheads in B,F,H indicate a non-neuronal cell. All cells in the NGN1 colony illustrated are neurons. No neurons have differentiated at this time point in control GFP-infected cultures (not shown). (I-P) Colonies from a similar experiment triple-labeled with antibodies to GFP (I,J), BRN3A (K,L) and PHOX2B (M,N); ‘Merge’ indicates superposition of the three labels.
