Fig. 9. Differences in cell cycle withdrawal-promoting activity and Notch sensitivity between NGN1 and MASH1 in NCSCs. (A-H) BrdU labeling in NGN1- or MASH1-infected colonies pulsed for 24 hours 3 days after infection. Colonies were labeled antibody to NF-M and the nuclear dye Topro-3 (A,B), anti-BrdU (C,D) and anti-GFP (E,F). Arrows in B,D,F,H indicate a neuronal cell, arrowheads indicate a non-neuronal cell (see Table 4 for quantification). (I-P) MASH1 is more sensitive than NGN1 to inhibition by Delta-Fc. Colonies were double-labeled with anti-GFP (green) and anti-NF-M (red). In K,L,O,P only the NF staining is shown for clarity. Arrows indicate process-bearing, NF-M+ neurons, arrowheads indicate NF-M– non-neuronal cells. Note that there are still some neurons in NGN1-infected colonies treated with Delta-Fc (P) (see Table 4 for quantification).
