Fig. 4. In situ localization of RNAs from floral homeotic genes in hen2-1 hua1-1 hua2-1 and hua1-1 hua2-1 developing flowers. (A-C) Hybridization with AP1 antisense probe. (A) hen2-1 hua1-1 hua2-1 at stage 4. (B) hen2-1 hua1-1 hua2-1 at stage 8-9 showing strong patches of AP1 expression in the gynoecium (arrowhead) and one third whorl organ (arrow). (C) Stage 8-9 hua1-1 hua2-1 from the same slide as (B) with weak expression of AP1 in the third and fourth whorls. (D-F) Hybridization with AP3 antisense probe. (D) Stage 3 hen2-1 hua1-1 hua2-1. (E) Stage 8-9 hen2-1 hua1-1 hua2-1 with patchy AP3 expression in the third whorl organs. (F) hua1-1 hua2-1 at stage 8-9. (G-I) Hybridization with PI antisense probe. (G) Stage 3 hen2-1 hua1-1 hua2-1. (H) hen2-1 hua1-1 hua2-1 at stage 8-9, note PI expression in only one of the visible third whorl organs (arrow). (I) Stage 8-9 hua1-1 hua2-1. (J-L) Hybridization with AG antisense probe. (J) Stage 3 hen2-1 hua1-1 hua2-1. (K) hen2-1 hua1-1 hua2-1 at stage 8 with low expression in the third and fourth whorl organs, note strong expression of AG in a stage 5 flower at the bottom of the panel. (L) Stage 8 hua1-1 hua2-1 flower from the same slide as (K). Scale bars, 50 µm (A,D,G,J), 100 µm (B,C,E,F,H,I,K,L).
