Fig. 5. Cloning of HEN2. (A) Mapping of the HEN2 locus to six BACs at the top of chromosome 2. Numbers represent the number of recombinant chromosomes in a total of 1390 chromosomes. The schematic below shows the basic structure of HEN2 with exons and introns represented by boxes and lines, respectively. The nucleotide substitution in exon 3 found in hen2-1 is marked. (B-D) Molecular complementation of hen2-1 hua1-1 hua2-1. (B) A flower from a hen2-1 hua1-1 hua2-1 plant containing HEN2g, showing rescue of the third whorl and gynoecium phenotypes. (C) A flower from a hen2-1 hua1-1 hua2-1 plant containing the vector alone. (D) A flower from a hen2-1 hua1-1 hua2-1 plant containing mHEN2g. Note lack of rescue in C and D.
