Fig. 8. In situ localization of HEN2 RNA in Ler inflorescences. (A-G) Antisense probe. (A) Low magnification of an Ler inflorescence showing strong expression of HEN2 in developing flowers of various stages. (B) An Ler inflorescence showing expression throughout the inflorescence meristem and stage 2 and 3 floral primordia. (C) A stage 3 flower with HEN2 expressed throughout. (D) A stage 5 flower, note HEN2 is no longer expressed in sepals. (E) A stage 7 flower. (F) A stage 8 flower. (G) A stage 10 flower, HEN2 is still expressed at a low level in the petals and gynoecium and strongly expressed in the developing ovules. (H,I) Hybridization with sense probe to Ler inflorescence (H) and stage 6 flower (I), showing lack of strong signal. Scale bars, 50 µm (B-F,I), 100 µm (A,G,H).
