Fig. 6. Slug overexpression increases the premigratory neural crest population in the trunk. (A) Trunk level of an embryo processed for rhoB and HNK-1 expression 30 hours after being electroporated with Slug at stage 12. No differences can be detected in the migratory neural crest of control and experimental sides although numerous electroporated GFP-expressing cells had migrated from the tube and can be seen in a similar pattern to that observed for HNK-1 (B). (D,E) A section of this embryo, confirming that the migratory population is similar in both sides. However, note an increase in the rhoB-expressing area in the right half of the spinal cord (E, brackets). This is better observed in a similar embryo only hybridised with rhoB (F). (C) Flat mount of the posterior hindbrain of an embryo electroporated at stage 9. The increase in rhoB-expressing crest cells both in the premigratory and in the migratory population can be better assessed in the section taken at the level of the dotted line (G).
