Fig. 6. Epidermis and cell structure in a single E2748 embryo (
60 mg seed, transition state), Toluidine Blue stained in A-D and transmission electron microscopy in E-G. (A) Young embryo at the early cotyledon stage with single epidermal cell layer having wild-type-like character (arrowheads). (B) As in A but note changed cell shape (arrowheads). (C) Highly vacuolated modified epidermal cells with altered surface. Dashed line indicates increasing morphological aberrations. (D) Modified epidermal cell with periclinal as well as anticlinal cell divisions (arrowheads). (E) Contact zone of modified epidermal cell and endosperm; note the tight contact zone with an increase in mitochondria and membrane structures. (F) Outer region of modified epidermal cell. (G) Inner region of modified epidermal cell (arrowhead indicates cell wall). ch, chloroplast; em, embryo; en, endosperm; er, endoplasmic reticulum; m, mitochondrion; n, nucleus; v, vacuole. Scale bars, 10 µm (A-D), 3 µm (E), 1.1 µm (F,G).
